Green Silver Nanoparticles From 10 Plants Essay
Biofabrication of green silver nanoparticles from 10 plants; an efficient approach to inhibit multi–drug resistant microbes and their biofilms.
Shariq Qayyuma and Asad U Khana
a Medical microbiology and molecular biology, Laboratory Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh 202002, India
Author for correspondence:
Email: asadukhan72@gmail.com
Phone No.:+91– 9837021912
Abstract:
Silver nanoparticles were biosynthesized via a green route using 10 different plants extracts and formed AgNPs were tested against drug resistant microbes and their biofilms. These nanoparticles were characterized using UV– vis spectroscopy and TEM which confirmed their synthesis, shape and size. FTIR clearly demonstrated the presence of the bio–groups on the surface of AgNPs. XRD confirmed the crystalline structure of AgNPs. TEM images were further analyzed using Image J software which showed that majority of particles were under 100 nm and majorly distributed over 1 to 60 nm size range. Their antimicrobial efficacy was checked against bacteria harboring antibiotic resistance genes like CTX–M–3, CTX–M–15, OXA–1, arm A, SHV–1 and NDM–1 in gram negative bacteria and fluconazole, amphotericin B and itraconazole resistant genes in fungus. Gram positive bacteria and fungi Candida albicans were inhibited at higher MICs values in comparison to Gran negative bacterial strains. The result indicated that these particles were antibacterial in nature without
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